Part:BBa_K2729011

DENV2 ancC-prM-E (Dengue Virus Serotype II)

Usage and Biology

Flavivirus Capsid (C), prM (precursor Membrane) and Envelope (E) are structural proteins responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle.

Characterization

The prM protein consists of an N-terminal pr domain followed by the M protein separated by a furin cleavage site.(15) The pr part of the protein consists mainly of β-strands, whereas the M portion consists of a linear structure followed by a mainly α-helical stem region and two transmembrane helices.

Figure 1: Result of the Electrophoresis

As shown in Figure 1, the band of DENV2 prME come around 2,000 bp.

Analysis

Our team successfully validated that the sequence was synthesized, and obtained enough amount of it for the transfection.

If it works well in the process of pseudo-virus production and also infection into host cells as expected, the fluorescence intensity can be observed robustly.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 946
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1397
  Illegal AgeI site found at 1108
  Illegal AgeI site found at 1450
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 349
  

Reference

- Membranotropic regions of the dengue virus prM protein